A method to study zhizosphere processes in thin soil layers of different proximity to roots |
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Authors: | Tara Singh Gahoonia Niels Erik Nielsen |
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Institution: | (1) Department of Forestry, University of Illinois, 61801 Urbana, IL, USA;(2) Department of Microbiology, University of Illinois, 61801 Urbana, IL, USA;(3) Present address: Department of Biochemistry, University of Victoria, V8W 2V2 Victoria, British Columbia, Canada |
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Abstract: | Frankia is the diverse bacterial genus that fixes nitrogen within root nodules of actinorhizal trees and shrubs. Systematic and ecological
studies of Frankia have been hindered by the lack of morphological, biochemical, or other markers to readily distinguish strains. Recently,
nucleotide sequence of 16 S RNA from the small ribosomal subunit has been used to classify and identify a variety of microorganisms.
We report nucleotide sequences from portions of the 16 S ribosomal RNA from Frankia strains AcnI1 isolated from Alnus viridis ssp. crispa (Ait.) Turrill and PtI1 isolated from Purshia tridentata (Pursh) DC. The number of nucleotide base substitutions and gaps we find more than doubles the previously reported sequence
diversity for the same variable regions within other strains of Frankia. |
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Keywords: | actinorhizal plants Alnus nitrogen-fixing bacteria Purshia rRNA sequences |
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