Variable compatibility of clonedAlnus glutinosa ecotypes against ineffectiveFrankia strains |
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Authors: | D Hahn M J C Starrenburg A D L Akkermans |
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Institution: | (1) Department of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands |
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Abstract: | Nodulation tests onin-vitro propagated clones ofAlnus glutinosa ecotypes (forest ecotype, pioneer ecotype) withFrankia strains originating from both ecotypes indicated differences in host-plant compatibility. Inoculated plants of the pioneer
ecotype clone were not infected by strains, that were unable to fix nitrogen in pure culture. Nodulation could only be induced
on the clone of the forest ecotype, but no nitrogen-fixing activity could be detected. Ultra-structural observations of the
nodules by SEM and TEM indicated that ineffectivity of these strains was correlated with the lack of vesicles in the infected
cells. Cells were only filled with hyphae: neither sporangia nor vesicles could be detected. In contrast, effective nodules
could be obtained on both alder clones after inoculation with an effective strain, showing normal development of vesicle clusters
in infected cells. In pure culture the ineffective strains produced no vesicles; sporangia were found only during early stage
of growth. The results demonstrate the existence ofFrankia strains which were either non-infective or ineffective on different clones ofAlnus glutinosa. |
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Keywords: | alder Alnus glutinosa clones compatibility ecotypes Frankia ineffectivity N2-fixation nodulation |
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