Evaluation of the use of a model rhizodeposition technique to separate root and microbial respiration in soil |
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Authors: | J Swinnen |
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Institution: | (1) DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, NL-6700 AA Wageningen, The Netherlands |
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Abstract: | A model rhizodeposition technique to estimate the root and microbial components of 14C soil/root respiration in pulse-labelling experiments is described. The method involves the injection of model rhizodeposits,
consisting of 14C-labelled glucose, root extract or root cell wall material, into the rooted soil of an unlabelled plant, simultaneously with
the pulse-labelling of a separate but similar plant with 14CO2. In a growth chamber experiment with 30 day old wheat and barley the contribution of direct root respiration to 14C soil/root respiration over a 26 day period after labelling was estimated 89–95%. Estimates of direct root respiration in
field-grown wheat and barley at different development stages in most cases accounted for at least 75% of 14C soil/root respiration over a 21 day period after labelling. The mineralization rate of injected 14C-glucose was positively correlated with the concentration of glucose-C established in soil. The use of the method in rhizosphere
carbon budget estimations is evaluated.
Communication No. 73 of the Dutch Programme on Soil Ecology of Arable Farming Systems.
Communication No. 73 of the Dutch Programme on Soil Ecology of Arable Farming Systems. |
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Keywords: | barley glucose microbial respiration pulse-labelling rhizodeposition root respiration wheat |
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