Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain |
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Authors: | Rongli Shi Hongwei Li Yiping Tong Ruilian Jing Fusuo Zhang Chunqin Zou |
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Institution: | (1) Department of Plant Nutrition, China Agricultural University, 2 West Yuanmingyuan Road, Haidian District, Beijing, 100094, China;(2) The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China;(3) Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081 Beijing, China |
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Abstract: | Zinc (Zn) is an essential micronutrient for human beings. However, Zn malnutrition has become a major problem throughout the
world. Wheat is the most important food crop in the world, therefore, developing Zn-enriched wheat varieties provides an effective
approach to reduce Zn malnutrition in human beings. The aim of this study was to understand the genetic control of grain Zn
density in wheat and hence, to provide genetic basis for breeding wheat with high grain Zn density using molecular approach.
A doubled haploid (DH) population developed from a cross between winter wheat varieties Hanxuan10 and Lumai 14 was used to
identify quantitative trait loci (QTLs) for Zn concentration and content in wheat grains. In addition, phosphorus (P) concentration
and content in wheat grain were also investigated to examine possible interactions between these two nutrients. The wheat
grains used in this study were harvested from the plants grown under normal condition in a field trial. We found the grain
Zn concentrations of the DH population varied from 25.9 to 50.5 mg/kg and the Zn content varied from 0.90 to 2.21 μg/seed.
The grain P concentrations of the DH population varied from 0.258 to 0.429 mg/kg, and the P contents varied from 0.083 to
0.186 mg/seed. A significant positive correlation was observed between Zn and P density in this experiment. The results showed
that both grain Zn and P densities were controlled by polygenes. Four and seven QTLs for Zn concentration and Zn content were
detected, respectively. All the four QTLs for Zn concentration co-located with the QTLs for Zn content, suggesting a possibility
to improve both grain Zn concentration and content simultaneously. Four and six QTLs for P concentration and P content were
detected, respectively. The two QTLs for grain Zn concentration on chromosomes 4A and 4D co-located with the QTLs for P concentration.
The four QTLs for grain Zn content on chromosome 2D, 3A and 4A co-located with the QTLs for P contents, reflecting the positive
correlations between the grain Zn and P density, and providing possibility of improving grain micro- and macronutrient density
simultaneously in wheat. In order to improve human health, the effect of P–Zn relation in grain on the Zn bioavailability
should also be considered in the future work. |
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Keywords: | Grain zinc density Grain phosphorus density QTLs Triticum aestivum L |
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