Some properties of carbohydrate and C4-dicarboxylic acid utilization negative mutants ofRhizobium leguminosarum biovarphaseoli strain P121 |
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Authors: | P J Lafontaine Carole Lafrenière H Antoun |
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Institution: | (1) Département des Sols, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, G1K 7P4, Québec, Canada;(2) Present address: Station de recherche, Agriculture Canada, P5N 2X9 Kapuskasing, Ontario, Canada |
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Abstract: | After NTG treatment of the very effective wild type strain P121 ofRhizobium leguminosarum biovarphaseoli, mutants defective in the utilization of sugars or organic acids were obtained. All the mutants nodulated the cultivar Goldie
ofPhaseolus vulgaris. The arabinose, fructose, glucose and pyruvate utilization mutants formed nodules similar in shape and size to the nodules
formed by the wild type strain. These mutants exhibited an acetylene reduction activity significantly lower than the activity
observed with the wild type strain. All the C4-dicarboxylic acid utilization mutatns, formed ineffective nodules that did not show a significant acetylene reduction activity.
The C4-dicarboxylic acids uptake system is apparently inducible in the free-living bacteria of strain P121. When P121 cells were
grown on glucose in the presence of 2.5 mM malate, the rate of glucose-dependent O2 consumption significantly decreased suggesting the presence of a catabolite repression-like phenomenon. Isolated bacteroids
of strain P121, under the experimental conditions used, were able to oxidize succinate, fumarate or malate but did not oxidize
pyruvate, glucose, fructose or sucrose. |
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Keywords: | french bean oxygem consumption Phaseolus vulgaris Rhizobium leguminosarum biovarphaseoli symbiotic effectiveness |
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