Cloning and characterization of two genes encoding sulfate transporters from rice (Oryza sativa L.)* |
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Authors: | Rosamond M Godwin Anne L Rae Bernard J Carroll Frank W Smith |
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Institution: | (1) CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, QLD, 4067, Australia;(2) School of Land and Food Sciences, University of Queensland, St Lucia, QLD, 4072, Australia;(3) School of Molecular and Microbial Sciences, University of Queensland, St Lucia, QLD, 4072, Australia |
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Abstract: | Two genes were isolated from a rice genomic library and the coding region of their corresponding cDNAs generated by RT-PCR. These single copy genes, designated ORYsa;Sultr1;1 and ORYsa;Sultr4;1, encode putative sulfate transporters. Both genes encode proteins with predicted topologies and signature sequences of the H+/SO42- symporter family of transporters and exhibit a high degree of homology to other plant sulfate transporters. ORYsa;Sultr1;1 is expressed in roots with levels of expression being strongly enhanced by sulfate starvation. In situ hybridization experiments revealed that ORYsa;Sultr1;1 expression is localized to the main absorptive region of roots. This gene probably encodes a transporter that is responsible for uptake of sulfate from the soil solution. In contrast, ORYsa;Sultr4;1 was expressed in both roots and shoots and was unresponsive to the sulfur status of the plant. The sequence of ORYsa;Sultr4;1 contains a possible plastid-targeting transit peptide which may indicate a role in transport of sulfate to sites of sulfate reduction in plastids. The role of the transporter encoded by ORYsa;Sultr4;1 is likely to be significantly different fromORYsa;Sultr1;1. These are the first reports of isolation of genes encoding sulfate transporters from rice and provide a basis for further studies involving sulfate transport. |
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Keywords: | chloroplast Oryza sativa plastid sulfate transporter sulfur assimilation |
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