Characterization and linkage mapping often sheep microsatellite markers derived from a sheep x hamster cell hybrid |
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| Authors: | J M Lumsden E A Lord G W Montgomery |
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| Institution: | AgResearch Molecular Biology Unit, Department of Biochemistry and Centre for Gene Research, University of Otago, PO Box 56, Dunedin, New Zealand |
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| Abstract: | A cosmid library has been constructed from a sheep x hamster cell hybrid containing sheep chromosome t1, rob (6;24). Clones containing sheep DNA were identified by hybridizing to a total sheep genomic DNA probe. Small fragments (<500 bp) containing (AC)n microsatellites were subcloned and sequenced. Ten microsatellite markers were characterized and six were mapped back to chromosomes 6 and 24. The remaining microsatellites mapped to chromosome 26, which was shown to be present in a small proportion of cells of the cell line. |
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| Keywords: | cosmid microsatellite sheep |
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