Characterization of the porcine transferrin gene (TF ) and its association with disease severity following an experimental Actinobacillus pleuropneumoniae infection |
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| Authors: | E Daniłowicz R Martinez‐Arias G Dolf M Singh I Probst B Tümmler D Höltig K‐H Waldmann G‐F Gerlach F Stanke T Leeb the FUGATO‐consortium IRAS |
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| Institution: | 1. Institute of Genetics, Vetsuisse Faculty, University of Berne, Bremgartenstrasse 109a, 3001 Berne, Switzerland;2. Present address: Department of Molecular Parasitology, Institute of Biology, Humboldt‐University Berlin, Philippstrasse 13, 10115 Berlin, Germany.;3. Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany;4. Clinical Research Group, OE6710, Hannover Medical School, Carl‐Neuberg‐Str. 1, 30625 Hannover, Germany;5. Swine Clinic, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany;6. Institute of Microbiology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany;7. IRAS members are: University of Veterinary Medicine Hannover, Hannover Medical School, Helmholtz Centre for Infection Research Braunschweig, Max Planck Institute for Molecular Genetics, Otto‐von‐Guericke University Magdeburg, IVD‐GmbH Hannover, LIONEX GmbH Braunschweig, RZPD GmbH Berlin, and the contributing scientists (in alphabetical order) Benga L, Bl?cker H, Dani?owicz E, Drungowski M, Gerlach GF, Herwig R, H?ltig D, Kahlisch D, Leeb T, Martinez‐Arias R, Naim HY, Pabst R, Probst I, Radelof U, Rehm T, Rothk?tter HJ, Singh M, Spalleck R, Stanke F, Strutzberg‐Minder K, Thies K, Tümmler B, Valentin‐Weigand P, Wagner F, Waldmann KH. |
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| Abstract: | Transferrin (TF)‐mediated provision of iron is essential for a productive infection by many bacterial pathogens, and iron‐depletion of TF is a first line defence against bacterial infections. Therefore, the transferrin (TF) gene can be considered a candidate gene for disease resistance. We obtained the complete DNA sequence of the porcine TF gene, which spans 40 kb and contains 17 exons. We identified polymorphisms on a panel of 10 different pig breeds. Comparative intra‐ and interbreed sequence analysis revealed 62 polymorphisms in the TF gene including one microsatellite. Ten polymorphisms were located in the coding sequence of the TF gene. Four SNPs (c.902A>T, c.980G>A, c.1417A>G, c.1810A>C) were predicted to cause amino acid exchanges (p.Lys301Ile, p.Arg327Lys, p.Lys473Glu, p.Asn604His). We performed association analyses using six selected TF markers and 116 pigs experimentally infected with Actinobacillus pleuropneumoniae serotype 7. The analysis showed breed‐specific TF allele frequencies. In German Landrace, we found evidence for a possible association of the severity of A. pleuropneumoniae infection with TF genotypes. |
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| Keywords: | Actinobacillus pleuropneumoniae APP association disease resistance pig TF transferrin |
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