Molecular characteristics of the porcine DLK1 and MEG3 genes |
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| Authors: | Li X P Do K T Kim J-J Huang J Zhao S H Lee Y Rothschild M F Lee C K Kim K S |
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| Institution: | College of Agriculture, Life and Environment Sciences, Chungbuk National University, Chungbuk 361-763, Korea;. School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea;. Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China;. Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011-3150, USA;. College of Life Sciences &Biotechnology, Korea University, Seoul 136-701, Korea |
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| Abstract: | Imprinted genes play important roles in embryo survival and postnatal growth regulation. The DLK1 and MEG3 (previously GTL2) genes are linked and reciprocally imprinted in several mammals, but their imprinting status is still unknown in pigs. In this study, we report polymorphisms, imprinting status and QTL analyses of the porcine DLK1 and MEG3 genes. Muscle and adipose DNA and RNA samples from 30-day-old animals generated with reciprocal crosses between the Korean native pig (KNP) and Yorkshire breeds were used to analyse DLK1 and MEG3 variation and expression. The samples exhibited paternal expression of DLK1 and maternal expression of MEG3 in pigs. These results indicated that the imprinting status of the DLK1 and MEG3 genes is conserved across mammalian species. By linkage analyses, we assigned the DLK1 and MEG3 genes to the telomeric region of SSC7. By QTL analyses, we confirmed a significant polar overdominance (POD) effect in DLK1 , which was previously detected for several growth traits in pigs. However, no significant POD effect was found with the MEG3 locus. |
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| Keywords: | DLK1 GTL2 imprinting MEG3 pig polar overdominance single nucleotide polymorphism |
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