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油樟悬浮培养及次生代谢产物的诱导
引用本文:魏琴,王丽,傅体华,周黎军.油樟悬浮培养及次生代谢产物的诱导[J].植物学通报,2008,25(5):591-596.
作者姓名:魏琴  王丽  傅体华  周黎军
作者单位:1. 宜宾学院西南特色经济植物保护与利用重点实验室,宜宾,644000
2. 四川农业大学,雅安,625014
基金项目:四川省教育厅资助项目,四川省科技厅资助项目
摘    要:以油樟(Cinnamomum longepaniculatum)叶片为外植体在附加6-BA和NAA不同激素浓度组合的MS培养基上筛选质地疏松、生长旺盛的愈伤组织,分别接种在MS、B5、WPM三种液体培养基中进行细胞悬浮培养,并检测诱导产生的次生代谢产物。结果表明:2mg·L^-16-BA+0.5mg·L^-1NAA能诱导质地疏松、生长旺盛的愈伤组织,B5基本培养基中细胞长势最好;愈伤组织在B5+2mg·L^-16-BA+0.5mg·L^-1NAA中悬浮培养,继代2次后形成均一的单细胞;从油樟悬浮培养物中检测出50%以上的成分是苯甲醇。

关 键 词:愈伤组织  油樟  次生代谢  悬浮培养

Studies of Cell Suspension Culture and Induction of Secondary Me-tabolism Products in Cinnamomum Iongepaniculatum
Qin Wei,Li Wang,Tihua Fu,Lijun Zhou.Studies of Cell Suspension Culture and Induction of Secondary Me-tabolism Products in Cinnamomum Iongepaniculatum[J].Chinese Bulletin of Botany,2008,25(5):591-596.
Authors:Qin Wei  Li Wang  Tihua Fu  Lijun Zhou
Institution:Qin Wei, Li Wang, Tihua Fu, Lijun Zhou(1.The Key Laboratory of Economic Plant Protection and Utilization in Southwest, Yibin College, Yibin 644000, China; 2.Sichuan Agricultural University, Ya'an 625014, China)
Abstract:Well-growing and friable calli from leaves of Cinnamomum longepaniculatum were screened on Murashige and Skoog (MS) medium with different plant growth regulators including 6-benzyladenine (6-BA) and 1 -naphthaleneacetic acid (NAA). Calli were cultured on MS medium, Gamborg medium (BS) and woody plant medium (WPM) through suspension culture, and secondary metabolism products were detected. Well-growing and friable-structured calli were induced on 2 mg·L^-16-BA + 0.5 mg·L^-1 NAA, and growth of calti was best on B5 medium in three liquid media. Single cells were formed on B5 medium with 0.2 mg·L^-1 6-BA + 0.5 mg ·L^-1 NAA after two periods of subcultivation. More than 50% benzenemethanol was detected in the secondary metabolism products.
Keywords:callus  Cinnamomum Iongepaniculatum  secondary metabolism  suspension culture
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