| cDNA-AFLP技术在植物表达分析上的应用 |
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| 引用本文： | 卢钢,曹家树.cDNA-AFLP技术在植物表达分析上的应用[J].植物学通报,2002,19(1):103-108. |
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| 作者姓名： | 卢钢 曹家树 |
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| 作者单位： | 浙江大学农业与生物技术学院,310029 |
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| 基金项目： | 国家基金资助项目。项目名称 :十字花科蔬菜直根膨大分子基础研究 ( 39870 5 0 9) |
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| 摘 要： | 阐述了一种mRNA指纹图谱技术 ,即cDNA_AFLP技术的原理和程序 ;该技术具有重现性高、准确可靠、效率高等特点 ,可广泛应用于植物遗传标记分析、基因表达特性研究以及分离植物基因等 ,具有良好的发展前景。
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| 关 键 词： | 扩增片段长度多态性 mRNA 指纹图谱技术 |
| 修稿时间： | 2001年3月12日 |
The cDNA-AFLP Technique and Its Application in Plants |
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| LU Gang,CAO Jia_Shu.The cDNA-AFLP Technique and Its Application in Plants[J].Chinese Bulletin of Botany,2002,19(1):103-108. |
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| Authors: | LU Gang CAO Jia_Shu |
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| Abstract: | A method of AFLP_based fingerprinting of mRNA named cDNA_AFLP was developed in recent years. In the present paper, the principle, procedure, advantages and defects of this technique were described. Because of its high reproducibility, accuracy and reliability, this technique is widely applied in plant research, for instance, analyzing differential expression, mapping of genetic loci and identifying genes. Its prospect was also discussed. |
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| Keywords: | AFLP mRNA Fingerprinting |
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