| 皱皮木瓜愈伤组织诱导与快速繁殖 |
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| 引用本文： | 范小峰,郭小强,肖朝霞,刘灵霞.皱皮木瓜愈伤组织诱导与快速繁殖[J].植物学通报,2009,44(6):725-727. |
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| 作者姓名： | 范小峰 郭小强 肖朝霞 刘灵霞 |
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| 作者单位： | 陇东学院生命科学系,庆阳,745000 |
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| 摘 要： | 以皱皮木瓜（Chaenomeles speciosa）当年生枝条的茎尖和茎段为外植体进行离体培养与快速繁殖。结果表明：芽萌发及愈伤组织诱导的最佳培养基是MS＋2.0mg·L^-1 6-BA＋1.0mg·L^-1NAA;愈伤组织分化最佳培养基是MS＋1.0mg·L^-1 6-BA＋0.05mg·L^-1NAA;芽增殖最佳培养基是MS＋1.0mg·L^-1 6-BA＋0.05-0.1mg·L^-1NAA,其增殖系数为6.59;最佳生根培养基是1/2MS＋1.0mg·L^-1IAA,生根率为86.7%;采用珍珠岩二步移栽,成活率可达90%以上。
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| 关 键 词： | 愈伤组织诱导 皱皮木瓜 快速繁殖 |
Callus Induction and Rapid Propagation of Chaenomeles speciosa |
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| Xiaofeng Fan,Xiaoqiang Guo,Zhaoxia Xiao,Lingxia Liu.Callus Induction and Rapid Propagation of Chaenomeles speciosa[J].Chinese Bulletin of Botany,2009,44(6):725-727. |
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| Authors: | Xiaofeng Fan Xiaoqiang Guo Zhaoxia Xiao Lingxia Liu |
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| Institution: | (Department of Life Sciences,Longdong University,Qingyang 745000,China ) |
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| Abstract: | This experiment used the current stem-segment with shoot apexes and segments as explants in callus induction and rapid propagation of Chaenomeles speciosa. The optimal medium for bud and callus induction was MS+2.0 mg-L~(-1)6-BA+1.0 mg.L~(-1) NAA, and the optimal propagation medium was MS+1.0 mg.L~(-1)6-BA+0.05-0.1 mg.L~(-1) NAA. The proliferation coefficient was 6.59. For callus differentiation, the optimal medium was MS+ 1.0 mg.L~(-1) 6-BA+ 0.05 mg.L~(-1) NAA, and for rooting, 1/2 MS+1.0 mg.L. IAA. The ratio of rooting was 86.7%, and that of survival was more than 90% when small intact plants were transplanted on perlite seedbeds. |
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| Keywords: | callus induction Chaenomeles speciosa rapid propagation |
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