| 桑树二倍体及人工诱导的同源四倍体遗传差异的AFLP分析 |
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| 引用本文： | 王卓伟,余茂德,鲁成.桑树二倍体及人工诱导的同源四倍体遗传差异的AFLP分析[J].植物学通报,2002,19(2):194-200. |
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| 作者姓名： | 王卓伟 余茂德 鲁成 |
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| 作者单位： | 农业部蚕桑学重点实验室,西南农业大学,重庆,400716 |
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| 基金项目： | 重庆市科委应用基础项目资助 (渝科计 1 999( 33)号 ) |
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| 摘 要： | 利用AFLP(AmplifiedFragmentLengthPolymorphism)分子标记技术 ,即扩增片段长度多态性 ,从DNA分子水平探讨二倍体桑 (2n =2X =2 8)与经秋水仙素诱变得到的同源四倍体桑 (2n =4X =5 6 )在遗传结构上的差异。根据对供试材料DNA多态性及遗传距离分析 ,认为经秋水仙素诱变得到的同源 4X与 2X相比在DNA分子遗传结构上产生一定程度的改变 ,在种内变异水平上 ,2X与同源 4X桑间的遗传差异小于种间差异。
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| 关 键 词： | 桑树 二倍体 同源四倍体 遗传差异 AFLP分析 |
| 修稿时间： | 2001年3月26日 |
The AFLP Analysis of Genetic Diversity between Diploid and Artificially Induced Homologous Tetraploid of Mulberry |
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| WANG Zhuo_Wei,YU Mao_De,LU Cheng.The AFLP Analysis of Genetic Diversity between Diploid and Artificially Induced Homologous Tetraploid of Mulberry[J].Chinese Bulletin of Botany,2002,19(2):194-200. |
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| Authors: | WANG Zhuo_Wei YU Mao_De LU Cheng |
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| Abstract: | AFLP molecular markers is used to analyze the genetic diversity between diploid and homologous tetraploid of mulberry chemically induced with by colchicines. According to the analysis of DNA polymorphism and genetic distance of assayed materials , the genetic diversity between diploid and homologous tetraploid is less than that of interspecies and is in the range of intraspecies diversity. It can be concluded that the DNA molecular genetic structure is changed by colchicines. |
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| Keywords: | Mulberry Diploid Homologous Tetraploid Genetic diversity AFLP analysis |
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