小黑杨花药培养植株转化胆碱氧化酶基因提高耐盐性

以小黑杨（Populus simonii×p．nigra）花药培养植株无菌苗叶片为外植体，通过根癌农杆菌（Agrobacterium tumefaciens）介导法将胆碱氧化酶基因（codA）导入小黑杨中，共获得4株转化株系，PCR扩增和Southern杂交检测结果全部呈阳性，表明codA基因已整合到小黑杨花药培养植株基因组中。荧光定量RT-PCR检测证明，codA基因在小黑杨花药培养植株中获得表达。耐盐实验结果显示，各转基因株系在0．6％的NaCl浓度下能够生长，而非转基因对照小黑杨受盐害严重，说明codA基因的导入提高了转基因植株的耐盐性。

Expression of Choline Oxidase Gene in Transformed Populus simonii x P.nigra Improves Salt Stress Tolerance

Choline oxidase gene （codA） was introduced into the haploid plants of Populus simonii x p. nigra byAgrobacteriummediated approach, with axenic leaves used as explants. PCR and Southern blot analysis confirmed four kanamycin-resistant transgenic plants, in which codA successfully integrated into the genome of Populus simonii x p. nigra haploid plants. Real-time quantitative PCR revealed that the transgene was expressed in the transgenic plants at different levels. Salt tolerance test demonstrated that the 4 transgenic plants could grow normally at 0.6% salinity, but nontransgenic plants suffered severely from the same salinity. Thus, the expression of codA gene improves the salt-stress tolerance of these transgenic poplar plants.