| 枸杞体细胞胚发生中DNA代谢动态的立体计量 |
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| 引用本文： | 戴若兰,张玮,邢更生,李杉,王亚馥.枸杞体细胞胚发生中DNA代谢动态的立体计量[J].植物学通报,2000,17(1):80-83. |
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| 作者姓名： | 戴若兰 张玮 邢更生 李杉 王亚馥 |
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| 作者单位： | 兰州大学干旱农业生态国家重点实验室,兰州 |
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| 基金项目： | 国家自然科学基金资助项目!编号:3 9770 3 1 75 |
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| 摘 要： | 本文以宁夏枸杞无菌苗叶片为材料，离体培养，并诱导体细胞胚胎发生。根据细胞形态计量学原理，应用数学图像处理软件计量由光学底片经Ａ／Ｄ转换成的数学图像中的ＤＮＡ大分子，对枸杞体细胞胚发生过程中ＤＮＡ分子的代谢动态进行量化分析。结果表明：在整个体细胞胚发和过程中ＤＮＡ代谢呈现动态变化。非胚性细胞与胚性细胞期的量化值分别为１．８２％和１．９１％；在二细胞胚、四细胞胚、多细胞胚时期ＤＮＡ缓慢增长，随着胚性愈
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| 关 键 词： | 枸杞 体细胞胚 DNA合成 立体计量 |
Quantitative
Stereologic Analysis of Changes in the Metabolic Kinesis of DNA During Somatic
Embryogenesis |
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| DAI Ruo_Lan ZHANG Wei XING Geng_Sheng LI Shan WANG Ya_Fu The State Key Laboratory of Arid Land Agro_ecology,Lanzhou University,Lanzhou.Quantitative
Stereologic Analysis of Changes in the Metabolic Kinesis of DNA During Somatic
Embryogenesis[J].Chinese Bulletin of Botany,2000,17(1):80-83. |
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| Authors: | DAI Ruo_Lan ZHANG Wei XING Geng_Sheng LI Shan WANG Ya_Fu The State Key Laboratory of Arid Land Agro_ecology Lanzhou University Lanzhou |
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| Institution: | DAI Ruo_Lan ZHANG Wei XING Geng_Sheng LI Shan WANG Ya_Fu The State Key Laboratory of Arid Land Agro_ecology,Lanzhou University,Lanzhou 730000) |
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| Abstract: | Using leaves of Lycium barbarum L. as explant to induce somatic embryogenesis. The DNA in somatic embryos of Lycium barbarum L. were analysed using quantitative stereological procedure formed from cell morphometry. The results indicated that the quantity of DNA changes during somatic embryogenesis. The quantitive value of DNA was 1.82% and 1.92% respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear_shaped embryonic stage keep high level at mature embryonic stage. We concluded that dynamic changes of DNA has close relationship with embryogenesis and cell reproduction. |
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| Keywords: | Lycium barbarum L Somatic embryogensis Metabolic kinesis of DNA Quantitative stereological analysis |
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