产藏红花素1(crocin)愈伤组织的诱导及其细胞系的筛选

对藏红花(cfocus sativus L)愈伤组织的诱导条件进行了优化.结果表明:Ms是藏红花芽愈伤组织的最佳诱导培养基,而B5是叶子和花愈伤组织的最佳培养基.藏红花芽、叶和花愈伤组织的最佳诱导温度分别是18℃、25℃和21℃.光照是叶子愈伤组织诱导的有利因素,但不利于芽和花愈伤组织的诱导.1.5～2.0 mg·L-1NAA和0.25 mg·L-6-BA是愈伤组织诱导的最佳激素组合.通过目视法和HPLC方法,从229株细胞系中筛选出细胞系Corml,其藏红花素1的含量是1 677 mg·g-,生长较快,且不易褐化.为采用植物细胞工程法解决藏红花素1资源短缺问题打下了基础.

Screening of Crocus sativus L.Callus Lines for Crocin Production

Induction conditions of Crocus sativus L. callus were optimized. MS medium was optimal for shoot callus induction while B5 medium was optimal for leaf and flower callus induction. 18 ℃, 25 ℃ and 21 ℃ were the optimal temperature for callus induction of shoot, leaf and flower, respectively.Light was positive factor for leaf callus induction but negative for shoot and flower induction.Naphthaleneacetic acid (NAA, 1.5-2.0 mg.L-1) and 6-benzyladenine (6-BA, 0.25 mg.L-1) were the optimal hormones for callus induction. Under the optimized conditions, the induction rate reached 60% for shoot callus, 80% for leaf callus, and 35% for flower callus. Using eyesight and HPLC methods, one callus line which gave rise to 1 677 mg.g-1 crocin and would not brown was obtained from 229 callus lines.