| 黄瓜子叶扩张与细胞分裂素的关系 |
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| 引用本文： | 马庆虎,侯林林.黄瓜子叶扩张与细胞分裂素的关系[J].植物学通报,1993,10(1):43-45. |
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| 作者姓名： | 马庆虎 侯林林 |
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| 作者单位： | 中国科学院植物研究所 北京100044
(马庆虎，侯林林，吴欣，唐志强)，中国科学院植物研究所 北京100044(宋艳茹) |
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| 摘 要： | BA(benzyl adenine)专一性地促进离体黄瓜子叶的扩张。为了研究 BA 的作用机理,我们采用间接 EIISA 和 HPLC 的方法测定了子叶扩张过程中内源玉米素(Z)和玉米素核苷(ZR)含量的变化。离体黄瓜(Cucumis sativus,津研4号)子叶用10mg/l 的 BA 培养,72小时之后,处理子叶鲜重的增加比对照高70%。Z+ZR 在 BA 处理的子叶中有大量的积累。结果表明 BA 可能诱发了黄瓜子叶中的细胞分裂素生物合成和代谢的某些基因。
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| 关 键 词： | 黄瓜 子叶 扩张 细胞分裂素 |
THE RELATIONSHTP BETWEEN THE EXPANSION OF CUCUMBER COTYLEDONS AND CYTOKININ BIOSYNTHESIS |
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| Ma Qing-hu Hou Lin-lin Wu Xin Tang Zhi-qiang Song Yan-ru.THE RELATIONSHTP BETWEEN THE EXPANSION OF CUCUMBER COTYLEDONS AND CYTOKININ BIOSYNTHESIS[J].Chinese Bulletin of Botany,1993,10(1):43-45. |
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| Authors: | Ma Qing-hu Hou Lin-lin Wu Xin Tang Zhi-qiang Song Yan-ru |
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| Abstract: | Benzyladenine (BA) specifically promotes the expansion of excisedcucumber cotyledons.To investigate the mechanism of BA action,the fluctuationof endogenous zeatin (Z) and zeatin riboside (ZR) level was determined byindirect-ELISA and HPLC during the expansion process.The excised cucumber(Cucumis sativus,var Jin-ian No 4) cotyledons were incubated with 10 mg/1of BA.Up to 72 hour,the fresh weight of the treated cotyledons increasedmore than 70% compared with that of control.large amount of Z+ZR wereaccumulated in the BA-treated cotyledons.This suggested that BA might triggerthe cytokinin biosynthesis gene (s) in the cotyledons. |
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| Keywords: | Cucumber cotyledons expansion benzyladenine cytokinin |
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