| 木本植物蛋白提取和SDS-PAGE分析方法的比较和优化 |
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| 引用本文： | 谷瑞升,刘群录,陈雪梅,蒋湘宁.木本植物蛋白提取和SDS-PAGE分析方法的比较和优化[J].植物学通报,1999,16(2):171-177. |
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| 作者姓名： | 谷瑞升 刘群录 陈雪梅 蒋湘宁 |
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| 作者单位： | 中国科学院植物研究所，北京林业大学森林生物实验中心 |
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| 基金项目： | 国家教委优秀青年教师基金 |
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| 摘 要： | 在几种木本植物上对４种常用蛋白提取方法、４种凝胶染色和脱色方法及影响蛋白定量和ＳＤＳＰＡＧＥ电泳的因素进行了比较研究，发现：利用改良丙酮沉降法提取蛋白，不仅提取效率高，而且杂质干扰少，得到的电泳结果，蛋白条带清晰，数量多；将常规凝胶染色和脱色液中的乙醇改用为甲醇可大大改善染色和脱色效果，获得了没有背景或背景很浅的电泳结果。通过研究确定了一套优化的适用于木本植物的蛋白提取、定量、凝胶制备、电泳、染色、脱色以及干胶制备的方法。利用这一优化方法对胡杨细胞盐胁迫蛋白进行了研究，发现了与高水平盐胁迫有关的６６ｋＤ蛋白和与盐胁迫和干旱胁迫均有关的２８ｋＤ蛋白，获得了满意的蛋白ＳＤＳＰＡＧＥ分析结果。
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| 关 键 词： | 木本植物，蛋白提取，十二烷基磺酸钠聚丙烯酰胺凝胶电泳 |
COMPARISON AND OPTIMIZATION OF THE METHODS ON PROTEIN EXTRACTION AND SDS PAGE IN WOODY PLANTS |
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| GU Rui Sheng,LIU Qun Lu,CHEN Xue Mei,JIANG Xiang Ning.COMPARISON AND OPTIMIZATION OF THE METHODS ON PROTEIN EXTRACTION AND SDS PAGE IN WOODY PLANTS[J].Chinese Bulletin of Botany,1999,16(2):171-177. |
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| Authors: | GU Rui Sheng LIU Qun Lu CHEN Xue Mei JIANG Xiang Ning |
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| Institution: | 1GU Rui Sheng 2LIU Qun Lu 2CHEN Xue Mei 2JIANG Xiang Ning 1 |
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| Abstract: | The methods of protein extraction and SDS PAGE were compared and optimized in woody plants. The improved steps included modified protein extraction, substitution of ethanol for methanol in the solution of staining and destaining. Satisfied results were achieved in the application of the optimized method to the protein analysis of several woody plants. 66 kD protein band related to salt stress and 28 kD protein band associated with osmotic stress were obviously showed out in the analysis for the suspensivly cultured cells stressed with NaCl of Populus euphratica. |
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| Keywords: | Woody plants Protein extraction SDS PAGE |
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