桂林小花苣苔离体快速繁殖技术

对抗结核植物桂林小花苣苔（Chiritopsis repanda var.guilinensis）进行离体培养与快速繁殖技术研究。结果表明：桂林小花苣苔叶片外植体的最适初代诱导培养基为MS＋0.5mg·L^-16-BA＋0.05mg·L^-1IBA,pH8.0;最适继代增殖培养基为MS＋0.1mg·L^-16-BA＋0.05mg·L^-1IBA,pH6.0,繁殖系数7.0/35天;最适生根培养基为1/2MS＋0.2mg·L^-1NAA,pH6.0,生根率为93.6%。模拟桂林小花苣苔自然生境,在春季对生根试管苗进行大棚移栽,成活率达90%。根据上述快繁技术,理论上每株试管苗每年可繁殖桂林小花苣苔种苗46万株。

Rapid Propagation in Vitro of Chiritopsis repanda var. Guilinensis

We investigated in vitro culture and rapid propagation of the antituberculosis plant Chiritopsis repanda var. guilinensis. The optimal medium for primary culture for bud induction and seedling regeneration from leaves was MS＋0.5 mg·L^-16-BA＋0.05 mg·L^-11BA （pH8.0）. The optimal medium for subculture for bud multiplication was MS＋0.1 mg·L^-1 6-BA＋0.05 mg·L^-11BA （pH6.0）; the proliferation coefficient was 7.0/35 d. The optimal rooting medium was 1/2MS＋0.2 mg.L-1NAA （pH6.0）, and the rooting rate was 93.6%. The rooting plantlets were transplanted in a greenhouse that simulated the natural distribution environment of Chiritopsis repanda var. guilinensis in spring; the survival rate was more than 90%. According to this rapid propagation technique, about 460 000 seedlings were reproduced from a germfree plantlet in a year.