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亚麻组织培养高频不定芽诱导体系
引用本文:张志扬,陈信波,张瑜,龙松华,高原,刘爱玲.亚麻组织培养高频不定芽诱导体系[J].植物学通报,2007,24(5):629-635.
作者姓名:张志扬  陈信波  张瑜  龙松华  高原  刘爱玲
作者单位:中国农业科学院麻类研究所 农业部麻类遗传改良与工程微生物重点实验室,中国农业科学院麻类研究所,农业部麻类遗传改良与工程微生物重点实验室,湖南农业大学作物基因工程湖南省重点实验室,中国农业科学院麻类研究所,农业部麻类遗传改良与工程微生物重点实验室,湖南农业大学作物基因工程湖南省重点实验室,湖南农业大学生物科学技术学院,长沙410006湖南农业大学作物基因工程湖南省重点实验室,长沙410128,长沙410006湖南农业大学作物基因工程湖南省重点实验室,长沙410128,长沙410128,长沙410006,长沙410128,长沙410128
基金项目:中国农业科学院杰出人才科研启动经费
摘    要:对适合南方地区冬季种植的纤用亚麻品种组织培养过程中基本培养基、激素配比、外植体材料的基因型和苗龄以及再生不定芽的生根条件进行了比较研究。结果表明,适合于亚麻白花品种组织培养的最佳培养基为YB1,不定芽诱导率可达98.50%。在此培养基上,白花、黑亚4号、K6531、K7697、HI026、HI045、I039和阿丽亚那下胚轴不定芽的诱导率分别为98.50%、98.50%、56.50%、42.47%、54.40%、0、27.13%和97.30%,平均出芽数为11.43、9.33、2.17、0.77、1.10、0、0.90和10.68。苗龄为7-10天的下胚轴最适于诱导不定芽,随苗龄增加,不定芽的诱导率呈下降趋势。RB5培养基最适于不定芽的生根,生根率达100%,平均生根数为15.3。实验还确定了亚麻对卡那霉素、氨苄青霉素和头孢霉素的抗性浓度阈值。

关 键 词:不定芽  下胚轴  亚麻  再生体系
收稿时间:2006-11-17
修稿时间:2007-02-16

Highly Efficient Adventitious Shoot Induction from Hypocotyl Explants in Linum usitatissimum L.
Zhiyang Zhang,Xinbo Chen,Yu Zhang,Songhua Long,Yuan Gao,Ailing Liu.Highly Efficient Adventitious Shoot Induction from Hypocotyl Explants in Linum usitatissimum L.[J].Chinese Bulletin of Botany,2007,24(5):629-635.
Authors:Zhiyang Zhang  Xinbo Chen  Yu Zhang  Songhua Long  Yuan Gao  Ailing Liu
Institution:1 Ministry of Agriculture Key Laboratory of Genetic Improvement and Engineering Microbiology for Bast Fiber Crops, Institute of Bast Fiber Crops, the Chinese Academy of Agricultural Sciences, Changsha 410006, China 2 Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China 3 College of Bio-science and Technology, Hunan Agricultural University, Changsha 410128, China
Abstract:Flax is an important economic crop for fiber and/or oil in China. An efficient flax plant regeneration system was established through optimization of basal medium, hormone combination, flax genotype and age of hypocotyl explants. The best medium for callus and adventitious shoot differentiation was YB1. Shoot induction rate of Baihua, Heiya NO.4, K6531, K7697, HI026, HI045, I039 and Aliyana was 98.50%, 98.50%, 56.50%, 42.47%, 54.40%, 0, 27.13%, and 97.30%, respectively, and mean shoot number per explant was 11.43, 9.33, 2.17, 0.77, 1.10, 0, 0.90, and 10.68, respectively. Hypocotyls taken from seedlings 7 to 10 days old showed the highest regeneration ability. RB5 medium was optimal for root formation of regenerated shoots, with 100% root induction and 15.3 roots per shoot. Kanamycin at 60 mg.L-1 inhibited callus and shoot induction completely; the effect of ampicillin and cefotaxime at the concentrations tested was not significant.
Keywords:adventitious shoot  hypocotyls  Linum usitatissimum L    plant regeneration
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