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钙对低温胁迫的烟草幼苗某些酶活性的影响
引用本文:张燕,方力,李天飞,姚照兵,冯永新,吴业池.钙对低温胁迫的烟草幼苗某些酶活性的影响[J].植物学通报,2002,19(3):342-347.
作者姓名:张燕  方力  李天飞  姚照兵  冯永新  吴业池
作者单位:1. 云南烟草科学研究院生物技术重点实验室,昆明,650106
2. 中国科学技术大学经济技术学院,合肥,230052
摘    要:用CaCl2 浸种处理烟草种子 ,研究了钙对烟草幼苗某些酶活性的影响。结果表明 :CaCl2 浸种能够提高烟草幼苗结合态钙和膜保护酶活性 ,降低膜透性和MDA(丙二醛 )含量。在低温胁迫条件下 ,Ca2 浸种处理的烟草幼苗SOD、CAT和POD等保护酶活性下降程度较未经处理的轻 ,细胞相对电导率低。恢复生长后 ,幼苗膜透性和保护酶活性恢复较快。CaM(钙调素 )特异性抑制剂CPZ(氯丙嗪 )能部分抑制Ca2 提高SOD、CAT和POD活性的作用

关 键 词:  烟草幼苗  低温胁迫  保护酶  抗冷性
修稿时间:2001年12月24

Effect of Ca2+ on Activities of Some Enzymes in Tobacco Seedlings under Cold Stress
ZHANG Yan,FANG Li,LI Tian_Fei,YAO Zhao_Bing,FENG Yong_Xin,WU Ye_Chi.Effect of Ca2+ on Activities of Some Enzymes in Tobacco Seedlings under Cold Stress[J].Chinese Bulletin of Botany,2002,19(3):342-347.
Authors:ZHANG Yan  FANG Li  LI Tian_Fei  YAO Zhao_Bing  FENG Yong_Xin  WU Ye_Chi
Institution:ZHANG Yan 1FANG Li 1LI Tian_Fei 2YAO Zhao_Bing 2FENG Yong_Xin 2WU Ye_Chi 1
Abstract:After submerging tobacco seeds with CaCl 2 solution, we studied the effect of Ca 2 on activities of certain enzymes in tobacco seedlings. It was shown that submerging tobacco seeds with CaCl 2 solution increased the content of combinative calcium in tobacco seedlings and the protective enzyme activity, and reduced the permeability of cell membrane and the content of MDA. Under the low temperature stress, the reduction of SOD, POD and CAT activities in the tobacco seedlings treated with Ca 2 was lower than that of control seedlings . The relative electric conductivity of cells in tobacco seedling treated with Ca 2 also reduced. The recovery of cell membrane permeability of tobacco seedlings treated with Ca 2 was faster than that of control seedlings after seedling growth resumed. CPZ, the special inhibitor of CaM, could partly restrain the function of Ca 2 in increasing the activities of SOD, CAT and POD.
Keywords:Calcium    Tobacco  seedlings    Chilling  stress    Protective  enzymes  Cold  tolerance
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