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刺五加叶中金丝桃苷含量的测定
引用本文:曹建国,赵则海,杨逢建,李庆勇,祖元刚.刺五加叶中金丝桃苷含量的测定[J].植物学通报,2005,22(2):203-206.
作者姓名:曹建国  赵则海  杨逢建  李庆勇  祖元刚
作者单位:东北林业大学森林植物生态学教育部重点实验室,哈尔滨,150040
基金项目:教育部重点实验室基金,教育部科学技术研究项目
摘    要:用高效液相色谱法测定了刺五加(Acanthopanax senticosus(Rupr.&Maxin)Harms)叶中金丝桃苷(hyperin)的含量。色谱柱为C18柱,流动相为甲醇和0.025mol L-1磷酸,比例为V甲醇:V0.025mol.L-1磷酸.=55:45(混合后用三乙胺调pH3.0 ̄3.2),检测波长360nm。样品用甲醇超声提取。结果表明,金丝桃苷峰形良好,与其他组分的色谱峰达到基线分离。加样回收率为104%,相对标准偏差RSD为5.38%。本研究为刺五加叶中金丝桃苷含量的测定建立了一种可靠的方法。

关 键 词:刺五加  金丝桃苷  高效液相色谱法
修稿时间:2003年12月31

Determination of Hyperin in the Leaves of Acanthopanax senticosus
CAO Jian-guo,ZHAO Ze-Hai,YANG Feng-jian,LI Qing-yong,ZU Yuan-Gang.Determination of Hyperin in the Leaves of Acanthopanax senticosus[J].Chinese Bulletin of Botany,2005,22(2):203-206.
Authors:CAO Jian-guo  ZHAO Ze-Hai  YANG Feng-jian  LI Qing-yong  ZU Yuan-Gang
Abstract:This paper describes the use of high performance liquid chromatography (HPLC) to determine the hyperin in the leaves of Acanthopanax senticosus on a C18 chromatogram column. Methanol:0.025 mol L-1 phosphoric acid solution (V/V, 55:45) was used as the mobile phase (the . mixture was adjusted with triethylamine to pH 3.0-3.2), and the detection wavelength was 360 nm. The sample was extracted with methanol by the ultrasonic extraction method. Hyperin content showed a symmetrical peak shape and reached the baseline separation with the peak content of other chemicals. The average recovery was 104% and the relative standard deviation (RSD) 5.38%. This study pro- vided evidence of an accurate and credible method of determining the content of hyperin in the leaves of A. senticosus.
Keywords:Acanthopanax senticosus  Hyperin  High performance liquid chromatography
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