| 利用毛细管电泳法分析甜菊糖苷的含量 |
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| 引用本文： | 邵寒娟,胡涌刚,丁亮,沈明山,陈睦传,张向红.利用毛细管电泳法分析甜菊糖苷的含量[J].植物学通报,2001,18(1):113-117. |
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| 作者姓名： | 邵寒娟 胡涌刚 丁亮 沈明山 陈睦传 张向红 |
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| 作者单位： | 1. 厦门大学生命科学院细胞生物学与肿瘤细胞工程教育部重点实验室,
2. 厦门大学化学系固体表面物理化学国家重点实验室, |
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| 摘 要： | 本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。根据实验结果，优化的电泳条件为60mmol/LTris-硼酸缓冲液(pH8.0)，柱温30℃，工作电压25kV。优化条件下，甜菊苷(Stevioside)迁移时间的R.S.D为0.45%(15次)，且在7.45×10
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| 关 键 词： | 低能离子注入 毛细管区带电泳法 甜菊 甜菊苷 糖料作物 |
| 修稿时间： | 1999年10月28 |
Analysis of the Content of
Stevia Sweeteners by Capillary Zone Electrophoresis |
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| SHAO Han-juan,HU Yong-Gang,DING Liang,SHEN Ming-Shan,CHEN Mu-zhuan,ZHANG Xiang-Hong.Analysis of the Content of
Stevia Sweeteners by Capillary Zone Electrophoresis[J].Chinese Bulletin of Botany,2001,18(1):113-117. |
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| Authors: | SHAO Han-juan HU Yong-Gang DING Liang SHEN Ming-Shan CHEN Mu-zhuan ZHANG Xiang-Hong |
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| Institution: | SHAO Han Juan 2HU Yong Gang 1DING Liang 1SHEN Ming Shan 1CHEN Mu Zhuan 1ZHANG Xiang Hong 1 |
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| Abstract: | This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation. According to the experimental results, the optimum separation conditions were selected as: 60 mmol/L Tris-tetraborate buffer of pH 8.0, 30 ℃ and 25 KV. Under optimum conditions, the R.S.D. of stevioside migration time for 15 runs was 0.45%. The detector response for stevioside was linear over the range of 7.45×10-5～1.74×10-2 mol/L (r=0.9994). Stevia main sweeteners cound be separated effectively in less than five minutes. This method was applied for determining the variation of stevia main sweeteners after low-energy ions implantation. |
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| Keywords: | Implantation of low_energy ions Capillary zone electrophoresis Stevioside rebaudiana Stevioside |
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