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| 麻竹花药培养及再生植株的获得 | |
| 引用本文： | 乔桂荣,李海营,蒋晶,孙宗修,卓仁英.麻竹花药培养及再生植株的获得[J].植物学通报,2010,45(1):88-90. |
| 作者姓名： | 乔桂荣 李海营 蒋晶 孙宗修 卓仁英 |
| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,富阳,311400;中国水稻研究所,杭州,310006 2. 中国林业科学研究院亚热带林业研究所,富阳,311400 3. 中国水稻研究所,杭州,310006 |
| 摘 要： | 以麻竹（Dendrocalamus latiflorus Munro）花药为材料, 于M8＋2 mg·L^–1 NAA ＋0.5 mg·L^–1 6-BA＋15 mg·L^–1 PAA＋7.5 mg·L^–1 STS＋500 mg·L^–1 CH＋100 mg·L^–1 proline＋100 mg·L^–1 glutamin＋5.4% maltose＋0.8% agar的诱导培养基上成功诱导出胚性愈伤组织, 在此培养基上继代可形成体胚并分化成苗, 初步建立了麻竹花药一步成苗的再生体系。 |
| 关 键 词： | 花药培养 麻竹 苯乙酸 |
Anther Culture and Plant Regeneration of Dendrocalamus latiflorus |
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| Guirong Qiao,Haiying Li,Jing Jiang,Zongxiu Sun,Renying Zhuo Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang ,China State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou ,China.Anther Culture and Plant Regeneration of Dendrocalamus latiflorus[J].Chinese Bulletin of Botany,2010,45(1):88-90. | |
| Authors: | Guirong Qiao Haiying Li Jing Jiang Zongxiu Sun Renying Zhuo Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang China State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou China |
| Institution: | Guirong Qiao1,2,Haiying Li1,Jing Jiang1,Zongxiu Sun2,Renying Zhuo1 1Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400,China 2State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China |
| Abstract: | Embryogenic callus was initiated from bamboo （Dendrocalamus latiflorus Munro） anthers cultured on M8 medium supplemented with 2 mg·L^–1 NAA, 0.5 mg·L^–1 6-BA, 15 mg·L^–1 phenylacetic acid （PAA）, 7.5 mg·L^–1 silver thiosulfate （STS）, 500 mg·L^–1 casein enzymatic hydrolysate （CH）, 100 mg·L^–1 proline, 100 mg·L^–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established. |
| Keywords: | anther culture Dendrocalarnus latiflorus Munro phenylacetic acid |
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