杨树的组织培养及其基因工程研究

杨树是林木基因工程的模式植物，在其组织培养过程中，试管苗的再生不仅与植株的基因型，年龄及组织的来源，状态等有关，还受许多外界因素如盐浓度及激素的种类与配比的影响，在杨树的转化过程中，DNA直接转移法和农杆菌介导法都有应用，但后者较为常用，组织培养及转化技术的日趋成熟，为杨树基因工程奠定了良好基础，本文对杨树组织培养，DNA转化方法及其基因工程进行了较为系统的概述。

Studies on Tissue Culture and Gene Engineering of Poplar

Poplar has been developed as a model in forestry genetic engineering. The regeneration of its plantlets not only depends on the genotype and age of the donor tree, the origin and physiological state of the explants, but also is influenced by environmental factors, such as the content of inorganic compounds and the kind and ratio of exogenous hormones. Both direct DNA transfer and Agrobacterium_mediated DNA transfer have been applied to poplar gene engineering. However, the application of Agrobacterium_mediated DNA transfer was more common than that of direct DNA transfer. Along with the development of tissue culture and DNA transformation techniques, genetic engineering of poplar has quickly been developed and widely applied. Tissue culture and genetic engineering of poplar are reviewed in this paper.