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光照对大豆叶片苯丙氨酸裂解酶(PAL)基因表达及异黄酮合成的调节
引用本文:谢灵玲,赵武玲,沈黎明.光照对大豆叶片苯丙氨酸裂解酶(PAL)基因表达及异黄酮合成的调节[J].植物学通报,2000,17(5):443-449.
作者姓名:谢灵玲  赵武玲  沈黎明
作者单位:[1]中国农业大学生物学院农业部植物生理生化重点开放实验室 [2]中国农业大学生物学院农业部植物生理生化重点开放实
基金项目:国家自然科学基金!( 3967_0 1 81 )
摘    要:异黄酮是大豆体内特别是种子中积累的一类重要的次生代谢产物,它具有特殊的生物效能。本实验在不同水平(RNA/酶/产物)上研究不同光照条件对大豆叶片异黄酮合成过程中的第一个关键酶苯丙氨酸氨基裂解酶(PAL)基因表达的影响。研究发现,在光照条件下pal表达量比黑暗条件下高,而且其影响程度与品种有关系,种子中异黄酮含量低的品种表现更敏感;其mRNA的合成受红光、蓝光、紫外光的促进,其中紫外光最有效;随着处

关 键 词:大豆  异黄酮  光照  PAL  基因表达  生物合成  调控
修稿时间:1999-09-21

Light Regulation of the Expression of PAL Gene in Soybean Leaves and Isoflavone Synthesis
XIE Ling_Ling,ZHAO Wu_Ling,SHEN Li_Ming,WTBZ.Light Regulation of the Expression of PAL Gene in Soybean Leaves and Isoflavone Synthesis[J].Chinese Bulletin of Botany,2000,17(5):443-449.
Authors:XIE Ling_Ling  ZHAO Wu_Ling  SHEN Li_Ming  [WTBZ]
Institution:XIE Ling_Ling ZHAO Wu_Ling SHEN Li_Ming [WT6BZ]
Abstract:Isoflavones are a group of important secondary metabolites accumulated in soybean plant ( Glycine max Merri) especially in seeds, and have a lot of important functions. The accumulation of isoflavones in soybean leaves can be induced by light. Our experiments aimed at the soybean phenylalanine ammonia_lyase (PAL) gene expression under different light conditions. It was found that expression of pal in light was higher than that in darkness. The effects which depend on different varieties was more sensitive to light in the variety which has lower contents of flavones in seeds. Red light (R), Blue light (B) and UV light (UV) increased PAL mRNA abundance, and UV was the most effective light. With the elongation of treatment time, the mRNA abundance for pal and activity of the enzyme increased. The isoflavones accumulation showed to decline with the UV time. This pattern was not consistent with that at the level of mRNA and the enzyme.
Keywords:Soybean  Isoflavone  Light  Phenylalanine ammonia_lyase (PAL)
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