Induced pluripotent stem cells from hair follicles as a cellular model for neurodevelopmental disorders |
| |
Authors: | Petit I Kesner N Salman Karry R Robicsek O Aberdam E Müller F J Aberdam D Ben-Shachar D |
| |
Institution: | a INSERM U898, Nice, Franceb University of Nice-Sophia Antipolis, Nice, Francec INSERTECH, B. Rappaport Faculty of Medicine and B. Rappaport Research Institute, Technion, Haifa, Israeld Laboratory of Psychobiology, Department of Psychiatry, Rambam Medical Center, B. Rappaport Faculty of Medicine and B. Rappaport Research Institute, Technion, Haifa, Israele Zentrum für Integrative Psychiatrie, Kiel, Germany |
| |
Abstract: | Disease-specific induced pluripotent stem cells (iPSC) allow unprecedented experimental platforms for basic research as well as high-throughput screening. This may be particularly relevant for neuropsychiatric disorders, in which the affected neuronal cells are not accessible. Keratinocytes isolated from hair follicles are an ideal source of patients' cells for reprogramming, due to their non-invasive accessibility and their common neuroectodermal origin with neurons, which can be important for potential epigenetic memory. From a small number of plucked human hair follicles obtained from two healthy donors we reprogrammed keratinocytes to pluripotent iPSC. We further differentiated these hair follicle-derived iPSC to neural progenitors, forebrain neurons and functional dopaminergic neurons.This study shows that human hair follicle-derived iPSC can be differentiated into various neural lineages, suggesting this experimental system as a promising in vitro model to study normal and pathological neural developments, avoiding the invasiveness of commonly used skin biopsies. |
| |
Keywords: | HF hair follicle iPSC induced pluripotent stem cells ORS outer root sheath HPLC high performance liquid chromatography |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|