The measurement of human endometrial prostaglandin production a comparison of two methods |
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Authors: | MJ Peek IS Fraser CA Phillips TM Resta PM Blackwell R Markham |
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Institution: | Departments of Obstetrics and Gynaecology and Pharmacology University of Sydney, New South Wales, 2006, Australia |
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Abstract: | Two methods for measuring human endometrial prostaglandin production were compared. Endometrial samples from eight patients were incubated over eight hours by a perifusion and a superfusion technique. The collected fractions were assayed by radioimmunoassay for PGE2 and PGF2α.There was no significant difference between the perifusion and superfusion methods for the pattern and amount of PGE2 and PGF2 production with time. Significantly higher production levels of PGE2 and PGF2α were found in secretory phase endometria than in proliferative phase endometria. Histological examination of the tissue specimens by light and electron microscopy showed that both methods caused gross tissue damage after eight hours experimentation. The superfusion method produced more morphological damage than the perifusion method. However, no tissue damage could be detected after one hour of incubation with either method.Over an eight hour period neither the perifusion nor the superfusion technique appears to be a good indicator of endometrial prostaglandin production. Either reflect the situation. |
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