Effect of 16,16-dimethyl prostaglandin-E2 given intra-arterially or intra-gastrically, on acid secretion by canine stomachs perfused ex vivo

Isolated whole canine stomachs, perfused ex vivo with homologous blood, were used for studying the effect of 16,16-dimethyl prostaglandin-E₂ (dmPGE₂) on gastric secretion. DmPGE₂ was administered either into the gastric artery or instilled intra-gastrically through an esophageal cannula. Histamine, infused intra-arterially, induced acid secretion of all ex vivo stomachs and this was significantly inhibited by intra-arterially or intra-gastrically administered dmPGE₂; volume of secretion, output of HCl and concentration of H⁺ were decreased and gastric peripheral vascular resistance was significantly reduced. The inhibitory action of dmPGE₂ continued after its administration was stopped.