The platelet-activating factor receptor antagonist L-659,989 inhibits phospholipase D activity |
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Authors: | Antonio Gómez-Muñoz Lori O’Brien Urs P Steinbrecher |
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Institution: | Division of Gastroenterology, Department of Medicine, The University of British Columbia, 3300-950 West 10th Ave., Vancouver, BC V5Z 4E3, Canada |
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Abstract: | The platelet-activating factor (PAF) receptor antagonist L-659,989 (±)-trans-2-(3-methoxy-5-methylsulfonyl-4-propoxyphenyl)-5-(3,4,5-trimethoxyphenyl)tetrahydrofuran)] has been reported to be a specific inhibitor of the PAF receptor and as such, it is widely used for assessment of PAF receptor mediated biological effects. We report here that L-659,989 may not be as specific as previously reported because it is also a potent inhibitor of phospholipase D activity. At concentrations of 30 μg/ml, L-659,989 inhibited basal and agonist-stimulated phospholipase D activity by about 55% and 70–100% respectively, through a mechanism that may involve the generation of intracellular ceramides. Another PAF receptor antagonist, WEB-2086, did not affect phospholipase D activity at concentrations up to 50 μg/ml. Either of these inhibitors when present at 20 μg/ml are reported to fully block the effects of PAF. Furthermore, L-659,989 directly inhibited the activity of bacterial PLD in vitro. These results indicate that caution is required in the interpretation of results derived from the use of L-659,989. |
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Keywords: | Phospholipase D Platelet activating factor receptor antagonist Macrophage DME medium Dulbecco’s modified Eagle’s medium PA phosphatidic acid PAF platelet activating factor PBS phosphate-buffered saline PLD phospholipase D PMA 4β-phorbol 12-myristate 13-acetate SM sphingomyelin |
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