Microculture of single protoplasts of Brassica napus |
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| Authors: | G Spangenberg H-U Koop R Lichter H G Schweiger |
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| Institution: | Max-Planck-Inst. für Zellbiologie, D-6802 Ladenburg bei Heidelberg, Federal Republic of Germany;Botanisches Inst. der Univ. Düsseldorf, D-4000 Düsseldorf, Federal Republic of Germany. |
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| Abstract: | Protoplasts of Brassica napus L. were cultured individually in a microdroplet system using a synthetic medium with survival rates of more than 70% and division frequencies of up to 65%. Microcallus formation occurred at frequencies of up to 50%. Factors affecting the survival and division of individually cultured protoplasts, such as composition and volume of culture medium, pH, buffering system, osmolarity and genotype, were analyzed. |
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| Keywords: | Callus formation hypocotyl protoplasts individual culture rapeseed |
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