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Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks
Authors:Adam J Rubin  Kevin R Parker  Ansuman T Satpathy  Yanyan Qi  Beijing Wu  Alvin J Ong  Maxwell R Mumbach  Andrew L Ji  Daniel S Kim  Seung Woo Cho  Brian J Zarnegar  William J Greenleaf  Howard Y Chang  Paul A Khavari
Institution:1. Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA;2. Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, CA 94305, USA;3. Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA;4. Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA;5. Department of Applied Physics, Stanford University, Stanford, CA 94305, USA;6. Chan Zuckerberg Biohub, San Francisco, CA 94158, USA;7. Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA;8. Veterans Affairs Palo Alto Healthcare System, Palo Alto, CA 94304, USA
Abstract:
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  • Keywords:single-cell genomics  CRISPR  pooled screens  epigenomics  ATAC-seq  chromatin accessibility
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