Topological linkage of circular DNA molecules promoted by Ustilago rec 1 protein and topoisomerase |
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Authors: | E B Kmiec P E Kroeger M J Brougham W K Holloman |
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Institution: | Department of Immunology and Medical Microbiology University of Florida College of Medicine Gainesville, Florida 32610 USA |
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Abstract: | We studied the formation of linked circular DNA molecules promoted by the combined action of rec 1 protein and type I topoisomerase of Ustilago maydis. When ATP was added as cofactor to reactions containing rec 1 protein, pairs of homologous circular DNA molecules became linked after addition of topoisomerase. Closed circular duplex molecules could be joined at homologous sites with circular single-stranded molecules or with other circular duplex molecules, provided that homologous single-stranded DNA fragments or RNA polymerase and nucleoside triphosphates were also added. Complexes formed were topologically linked through regions of heteroduplex DNA. When the analog adenylyl-imidodiphosphate was substituted for ATP, nonhomologous pairs of circular DNA molecules became linked. |
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