A panoramic view of yeast noncoding RNA processing |
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Authors: | Peng Wen Tao Robinson Mark D Mnaimneh Sanie Krogan Nevan J Cagney Gerard Morris Quaid Davierwala Armaity P Grigull Jörg Yang Xueqi Zhang Wen Mitsakakis Nicholas Ryan Owen W Datta Nira Jojic Vladimir Pal Chris Canadien Veronica Richards Dawn Beattie Bryan Wu Lani F Altschuler Steven J Roweis Sam Frey Brendan J Emili Andrew Greenblatt Jack F Hughes Timothy R |
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Institution: | Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada. |
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Abstract: | Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA. |
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