Identification and characterization of<Emphasis Type="Italic"> Fugu</Emphasis> orthologues of mammalian interleukin-12 subunits |
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Authors: | Email author" target="_blank">Yasutoshi?YoshiuraEmail author Ikunari?Kiryu Atsushi?Fujiwara Hiroaki?Suetake Yuzuru?Suzuki Teruyuki?Nakanishi Mitsuru?Ototake |
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Institution: | (1) Immunology Section, National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, 519-0423 Mie, Japan;(2) Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Maisaka, Hamana, 431-0211 Shizuoka, Japan;(3) Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, 252-8510 Kanagawa, Japan |
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Abstract: | We have isolated and characterized cDNAs and genes for pufferfish, Fugu rubripes,
(Fugu) orthologues of mammalian interleukin (IL)-12 subunits (IL-12 p35 and IL-12 p40). The deduced amino acid sequences of the Fugu IL-12 subunits showed homology with mammalian IL-12 subunits (p35: 50.4–58.0% similarity; p40: 51.2–55.4% similarity). Phylogenetic analysis confirmed that Fugu IL-12 p35 and p40 genes cluster with their mammalian counterpart lineages. The genomic organization of each of the
Fugu IL-12 subunit genes is similar to that of the corresponding mouse IL-12 subunit genes, although the Fugu genes are very compact due to small intron size. Comparative genomic analysis showed conserved syntenies within the IL-12 p35 and p40 regions between Fugu and human, indicating that the Fugu IL-12 p35 and p40 genes are orthologues for mammalian IL-12 p35 and p40 encoding genes, respectively. Expression of IL-12 p35 mRNA was observed in lymphoid tissues and several non-lymphoid tissues, while expression of IL-12 p40 mRNA was constitutive and nearly ubiquitous. In the spleen and head kidney, expression of IL-12 p35 was induced by polyriboinosinic polyribocytidylic acid poly(I:C)] and not by lipopolysaccharide (LPS), while expression of IL-12 p40 was constitutive and unresponsive to both poly(I:C) and LPS. These results indicate that IL-12 levels are regulated by production of IL-12 p35 mRNA and suggest that IL-12 in fish may be involved in antiviral defense. This is the first report of the identification and characterization of IL-12 subunit cDNAs and genes in a non-mammalian vertebrate.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers AB096265, AB096266, AB096267, and AB096268. |
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Keywords: | Fugu Cytokine IL-12 Synteny Comparative immunology |
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