Molecular characterization of major histocompatibility complex class 1 (MHC-I) from squirrel monkeys (<Emphasis Type="Italic">Saimiri sciureus</Emphasis>) |
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Authors: | Hervé?Pascalis Jean-Michel?Heraud Rolf?Fendel Anne?Lavergne Email author" target="_blank">Mirdad?KazanjiEmail author |
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Institution: | (1) Laboratoire de Rétrovirologie, Institut Pasteur de la Guyane, 23 avenue Pasteur, BP 6010, 97306 Cayenne, French Guiana;(2) Centre de Primatologie, Institut Pasteur de la Guyane, 97306 Cayenne, French Guiana |
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Abstract: | Little is known about the major histocompatibility complex (MHC) class 1 in squirrel monkeys (Saimiri sciureus). We cloned, sequenced and characterized two alleles and the cDNA of the coding region of MHC class 1 in these New World monkeys. Phylogenetic analyses showed that these sequences are related to HLA class 1 genes (HLA-A and HLA-G). The structure and organization of one of the two identified clones was similar to that of a class 1 MHC gene (HLA-A2). All the exon/intron splice acceptor/donor sites are conserved and their locations correspond to the HLA-A2 gene. The sequences of the newly described cDNAs reveal that they code for the characteristic class 1 MHC proteins, with all the features thought necessary for cell surface expression. Typical sequences for the leader peptide, 1, 2, 3, transmembrane and cytoplasmic domains were found.The nucleotide sequence data reported in this paper have been submitted to the GenBank database and have been assigned the accession numbers AJ438576 (Sasc-G*31), AJ438577 (Sasc-G*25), AY282760 (Sasc-G*03), AY282761 (Sasc-G*04) and AY282762 (Sasc-G*05). Sequences were named as recommended by Klein and co-workers (1990) |
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Keywords: | Squirrel monkey MHC class 1 gene cDNA Phylogenetic analysis |
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