Anti-MHC immunity detected prior to intentional alloimmunization |
| |
Authors: | Radka Černý-Provazník Peter van Mourik Jacqueline Limpens Tine Leupers Dr Pavol Iványi |
| |
Institution: | (1) c/o Publication Secretariat, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, P. O. Box 9406, 1006 AK Amsterdam, The Netherlands;(2) Present address: Max-Planck-Institut für Biologie, Abteilung Immungenetik, Corrensstraße 42, D-7400 Tübingen, Germany |
| |
Abstract: | Cell fusion was performed between spleen cells from young BALB/cBy (H-2
d) mice which have never been immunized and SP2/0 mouse plasmacytoma cells. A monoclonal H-2-specific cytotoxic IgM antibody was obtained (By-1) which detected a new public biregional H-2 specificity, H-2.m210. The mcAb By-1 reacted strongly with H-2Kd, Dd, and H-2s antigens, gave weak cross-reactions with H-2Kk, Dq, H-2r, and H-2v antigens and was negative with H-2b, H-2f, H-2p, and H-2Ld antigens. A polymorphic reaction pattern was also observed on a panel of lymphocytes from B 10.W strains. The intriguing finding on this reaction pattern was the reactivity on H-2d cells, including the syngeneic BALB/cBy and truly autologous cells. As shown by capping and immunoprecipitation experiments on H-2d cells and by studies on H-2d-transfected mouse L cells, the target molecules for McAb By-1 were H-2Kd and H-2Dd molecules. The BALB/cBy mouse, from whose spleen cells the McAb By-1 was obtained, survived after the fusion experiment, and serum was examined for the presence of cytotoxic H-2-specific antibodies during the rest of its life. At the time of the fusion, no autoreactive serum antibodies were found, but about 4 months later, we found in the serum of this mouse autoreactive H-2-specific cytotoxic IgM antibodies. The serum antibodies followed the same reaction pattern as that of the McAb By-1. As far as we know, this is the first report of autoreactive H-2-specific antibodies in serum of a mouse which has never been immunized and of the first natural autoreactive H-2-specific monoclonal antibody.Abbreviations McAb
monoclonal antibody
- MHC
major histocompatibility complex
-
H-2
major histocompatibility complex of mice
- CTLs
cytotoxic T cells
- FMF
flow microfluorometry
- FITC
fluorescein isothiocyanate
- LPS
lipopolysaccharide W.E. coli 0111:134
- PBS
phosphate-buffered saline
- Iodogen
1,3,4,6,-tetrachloro-3,6-diphenylglycoluril
- GAMIg
goat-antimouse immunoglobulin
- Staph-A
Staphylococcus aureus Cowan I |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|