Expression of theQ8/9
d
gene by T cells of the mouse |
| |
| Authors: | Akihiro Matsuura Rene Schloss Fung-Win Shen Jwu-Sheng Tung Stephen W Hunt III Douglas A Fisher Leroy E Hood Edward A Boyse |
| |
| Institution: | (1) Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021 New York, NY, USA;(2) Department of Medicine, Division of Rheumatology and Immunology, University of North Carolina, 27599 Chapel Hill, NC, USA;(3) Developmental Biology Center, University od California at Irvine, 92717 Irvine, CA, USA;(4) California Institute of Technology, 91125 Pasadena, CA, USA;(5) Present address: Sapporo Medical College, South-1, West-17, Chuoku, 060 Sapporo, Japan;(6) Present address: Tampa Bay Research Institute, 10900 Roosevelt Boulevard North, 33716 St. Petersburg, FL, USA;(7) Present address: Merck, Sharp and Dohme Research Labs, 07065 Rathway, NJ, USA;(8) Present address: Department of Microbiology and Immunology, University of Arizona, Health Sciences Center, 85724 Tucson, AZ, USA |
| |
| Abstract: | TheQ genes, specifying Qa antigens and situated in the extended part of the major histocompatibility complex (MHC) of the mouse,
comprise a subgroup of MHC class I genes whose significance and function are still largely unknown. In screening a cDNA library
made from the BALB/c inducer T-cell line Cl.Ly1-T1, we isolated 11 clones representingQ8/9, but none representingQ6 orQ7. Confirmatory evidence is given that theQ8/9 gene originated from fusion of the 5′ region of theQ8 gene with the 3′ region of theQ9 gene at a recombination site or hot spot in the vicinity of intron 4. Contrary to previous impressions thatQ8/9 is an inert pseudogene, we find that theQ8/9 gene can be functional and encode a Qa-2,3 antigen. One variety of the 11 Q8/9 clones isolated lacked exon 5, which encodes
the transmembrane domain of class I glycoproteins, and thus may account for secretion of a soluble form of Qa-2,3 antigen
thought to be released by activated T cells. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
