Chromosome segregation and expression of rat immunoglobulins in rat/mouse hybrid myelomas |
| |
| Authors: | J Schröder K Autio J M Jarvis C Milstein |
| |
| Institution: | (1) Folkhälsan Institute of Genetics, P.O.Box 819, SF-00101 Helsinki 10, Finland;(2) MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH Cambridge, England |
| |
| Abstract: | The chromosome segregation of hybrid myelomas from a fusion of rat immunized spleen cells and the mouse myeloma P3-X63-Ag8 has been analysed. Chromosome loss appears to be nonrandom. Most mouse chromosomes are retained. The rat chromosomes are preferentially lost but a few — particularly 1–5 and 13-are rarely lost. The specific retention of some rat chromosomes explains the stability of rat Ig expression in mouse-rat hybrids. Correlation of chromosome loss and retention and loss of rat heavy and light chains leads us to propose chromosome 14 as coding for the heavy chain. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
