Mapping of antibody specificities to V
H gene families |
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Authors: | Garnett Kelsoe Robert Miceli Jan Cerny Dan H Schulze |
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Institution: | (1) Department of Microbiology, University of Texas Medical Branch, 77550 Galveston, TX, USA |
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Abstract: | V
H gene segments represent the products of the repeated duplication and subsequent diversification of a primordial V gene element. It is widely assumed that natural selection, operating via pathogens, has played the dominant role in this process. Here, we screen some 3.7 × 104 C
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colonies of mitogen-activated B cells for the production of antibodies specific for phosphorylcholine or hen egg lysozyme and expression of the V
H X-24, S107, Q52, or J558 gene families. These gene families were expressed at frequencies proportional to their genomic complexity among both unselected and antigen-specific C
+
colonies. Thus, the capacity to encode equivalent antibody-combining sites is dispersed uniformly among V
Hfamilies. This result suggests that individual V
H genes have not evolved to address specific antigens. |
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Keywords: | |
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