| 褪黑素对FSH诱导的小鼠卵母细胞体外成熟的影响 |
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| 引用本文： | 汪建红,曾献春,吕海英.褪黑素对FSH诱导的小鼠卵母细胞体外成熟的影响[J].生物学杂志,2005,22(1):17-19. |
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| 作者姓名： | 汪建红 曾献春 吕海英 |
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| 作者单位： | 新疆师范大学生命与环境科学技术学院,乌鲁木齐,830054 |
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| 基金项目： | 国家杰出青年基金资助项目(编号:30025032) |
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| 摘 要： | 通过次黄嘌呤(HX)阻滞、FSH诱导体外培养模型研究了褪黑素(MT)对小鼠卵母细胞成熟的影响,探讨褪黑素(MT)是否影响小鼠卵母细胞的体外成熟。0.1mg/mL和0.02mg/mL两有效浓度的MT能显著抑制促性腺激素(FSH)诱导的HX阻滞的CEOsGVBD的发生(P<0.05),对PBl的排出虽有一定的抑制作用,但没有统计学意义;MT和次黄嘌呤(HX)对CEOs的自发成熟有协同抑制作用(P<0.01),但在裸卵(DO)自发成熟的阻滞中没有协同效应。MT是调节哺乳动物卵母细胞成熟的重要激素之一,其作用机制可能是通过卵丘细胞实现的。
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| 关 键 词： | 褪黑素 体外成熟 生发泡破裂 第一极体 小鼠 |
| 文章编号： | 1008-9632(2005)01-0017-03 |
| 修稿时间： | 2004年7月14日 |
Effect of metlatonin on mouse oocyte maturation induced by FSH in vitro |
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| WANG Jian_hong,ZENG Xian_chun,LU Hai_ying.Effect of metlatonin on mouse oocyte maturation induced by FSH in vitro[J].Journal of Biology,2005,22(1):17-19. |
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| Authors: | WANG Jian_hong ZENG Xian_chun LU Hai_ying |
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| Abstract: | To study whether metlatonin could effect oocyte maturation of mouse in vitro mouse oocytes were cultured in HX-medium supplemented with FSH,and the effects of MT on meiotic maturation of mouse oocyte were examined.The result showed:(1)MT inhibited the effect of FSH on resumption of meiosis,but no effect on the formation of PB_1.(2)MT and HX had additive effects on spontaneous oocyte maturation in CEO,but not in DO.MT was able to affect mouse oocyte maturation and the regulation mechanisms might be related to cumulus cells. |
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| Keywords: | metlatonin maturation germinal vesicle breakdown first polar body mouse |
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