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西伯利亚鲟嗜水气单胞菌与其它3株气单胞菌间的免疫交叉反应
引用本文:姜有声,杨倩,杨先乐,吕利群,邱军强.西伯利亚鲟嗜水气单胞菌与其它3株气单胞菌间的免疫交叉反应[J].生物学杂志,2012,29(1):30-32.
作者姓名:姜有声  杨倩  杨先乐  吕利群  邱军强
作者单位:1. 上海海洋大学,水产与生命学院,国家水生动物病原库,上海201306
2. 上海海洋大学,水产与生命学院,国家水生动物病原库,上海201306;复旦大学上海医学院,上海,200032
基金项目:现代农业产业技术体系建设专项资金项目,淡水鱼类出血性疾病综合防治技术集成示范项目,上海高校选拔培养优秀青年教师科研专项基金
摘    要:采用间接免疫荧光技术分析了西伯利亚鲟细菌性败血症致病菌嗜水气单胞菌(Aeromonas hydrophlia)X1菌株、豚鼠气单胞菌(Aeromonas caviae)XL2-T菌株、致病性温和气单胞菌(Aeromonas sobria)W1菌株与无致病性嗜水气单胞菌(Aeromonas hydrophlia)M3菌株等水产养殖主要病原菌与抗血清之间的免疫交叉反应。结果显示具有致病性的同属菌株X1菌株、XL2-T菌株、W1菌株交叉反应程度较大,说明这3株菌表面存在较多相同抗原决定簇。而无致病性菌株M3与其他3株致病性菌株免疫交叉反应程度较小。

关 键 词:嗜水气单胞菌  豚鼠气单胞菌  温和气单胞菌  抗血清  免疫交叉

The immunocross-reaction between pathogenic Aeromonas hydrophila isolated from Siberian sturgeon Acipenser baerii and other three Aeromonas strains
JIANG You-sheng,YANG Qian,YANG Xian-le,LV Li-qun,QIU Jun-qiang.The immunocross-reaction between pathogenic Aeromonas hydrophila isolated from Siberian sturgeon Acipenser baerii and other three Aeromonas strains[J].Journal of Biology,2012,29(1):30-32.
Authors:JIANG You-sheng  YANG Qian  YANG Xian-le  LV Li-qun  QIU Jun-qiang
Institution:JIANG You-sheng, YANG Qian1 , YANG Xian-le1 , LV Li-qun1 , QIU Jun-qlang1, 2 ( 1. College of Fisheries and Life Science, Shanghai Ocean University, State Collection Center of Aquatic Pathogen, Shanghai 201306; 2. Shanghai Medical College, Fudan University, Shanghai 200032, China)
Abstract:The immunological characteristics of four Aeromonas strains like pathogenic bacteria strain X1 (Aeromonas hydrophlia )isola- ted from Siberian sturgeon (Acipenser baerii) suffering with bacterial septicaemia, pathogenic bacteria strain XL2-T strain (Aeromonas caviae ) isolated from the sturgeon septicemia, pathogenic strain W1 (Aeromonas sobria ) and non-pathogenic M3 strain (Aeromonas hydrophlia), were detected through the cross-reactions between four Aeromonas strains and antiserums by indirect immunofluoresence assay. The results showed that the pathogenic X1 strain, XL2-T strain and W1 strain had strong cross reactions, and weak cross reactions were found on non-pathogenic M3 strain with other three pathogenic strains.
Keywords:Aeromonas hydrophlia  cross reaction  indirect immunofluoresence assay
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