| 蝴蝶兰的组织培养研究 |
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| 引用本文： | 秦凡,周吉源.蝴蝶兰的组织培养研究[J].生物学杂志,2003,20(3):19-21. |
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| 作者姓名： | 秦凡 周吉源 |
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| 作者单位： | 华中师范大学生命科学学院,武汉,430079 |
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| 摘 要： | 探讨蝴蝶兰的组织培养技术和方法。实验表明：改良型M1培养基诱导原球茎有良好的效果，M1 BA5．0mg/L对花梗芽的诱导最合适，Ml BA3．0mg／L对茎尖诱导原球茎最合适。降低分裂素浓度后，原球茎可以分化成完整植株，壮苗阶段以改良型M2 BA0．1mg/L NAA0．5mg／L效果较佳。移栽基质选用水苔，成活率高。
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| 关 键 词： | 蝴蝶兰 组织培养 原球茎 |
| 文章编号： | 1008-9632(2003)03-0019-03 |
| 修稿时间： | 2002年10月8日 |
A study on tissue culure of Phalaenopsis |
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| QIN Fan,ZHOU Ji-yuan.A study on tissue culure of Phalaenopsis[J].Journal of Biology,2003,20(3):19-21. |
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| Authors: | QIN Fan ZHOU Ji-yuan |
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| Abstract: | The preliminary results of tissue culture of Phalaenopsis were reported.The results showed that M 1 medium has the positive effects on inducing the PLB(protocorm-like body).The best suitable medium for PLB inducement form stem opex was modified medium M 1+BA5.0mg/L.The best suitable medium for bud inducement form rachis was modified medium M 1+BA5.0mg/L.The PLB may differentiate into plants after the reducing of the cytokinin. At the prime stage the M 2medium supplemented with BA0.1mg/L and NAA0.5mg/L juice performed well. The optimal transplantation medium is water plant and the survival rate reach high. |
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| Keywords: | Phalaenopsis tissue culture protocom-like body |
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