大黄鱼Flotillin-1基因分子特征分析

浮舰蛋白-1(Flotillin-1)是属于SPFH家族的蛋白,是重要的脂筏标志性蛋白.在构建大黄鱼(Larimichthys crocea)肌肉组织cDNA文库的基础上,克隆了Flotillin-1基因,并进一步扩增出内含子.克隆到的序列全长为2497 bp,其中编码区1194 bp,编码397个氨基酸.生物信息学分析大黄鱼Flotillin-1有5类20个功能位点,存在2次跨膜结构,N端和C端都位于细胞膜内.大黄鱼Flotillin-1氨基酸序列具有非常高的保守性,与大西洋鲑和斑马鱼的同源性都在80%以上.在组织中的表达也非常广泛,其中在脾中的表达最强.

Molecular characterization of Flotillin-1 gene in Larimichthys crocea

Flotillin-1,one member of the SPFH protein family,is an important marker protein for lipid raft.On the basis of constructing the full length cDNA library from the skeletal muscle of Larimichthys crocea,flotillin-1 gene was cloned and sequenced.The intron sequences were also identified by PCR and sequencing.The acquired flotillin-1 gene was 2497 bp in length,and the coding sequence was 1194 bp in length,which encodes 397 amino acids.The bioinformatics analysis showed that flotillin-1 of Larimichthys crocea had twenty functional sites and had two hydrophobic transmembrane spanning domains.Both its N-and C-termini were located in the interior of cell membrane.The flotillin-1 amino acid sequence was highly conserved,and the similarities among Salmo salar,Danio rerio,and Larimichthys crocea were over 80%.The flotillin-1 gene was expressed in various tissues,with the highest expression in spleen,highly in brain,liver,eye,kidney and gill.