使用林鹳微卫星引物对东方白鹳基因组DNA进行交叉扩增

利用林鹳11个微卫星位点的引物对东方白鹳进行交叉扩增。经过PCR体系的优化,在11个位点中有6个得到清晰的扩增条带,其余5位点得不到确切的扩增产物。对上述6个位点的扩增产物进行克隆测序分析,发现其中4个位点上的扩增产物含有微卫星重复序列,而另外两个位点中无重复单元。通过基因分型对上述4个微卫星位点进行多态性分析后发现其中的WSμ13,WSμ17位点分别为高度多态和中度多态位点,而另外两个位点则无多态性。同时还对影响交叉扩增结果成功率及微卫星位点多态性的因素进行了分析和总结。

Pilot study on the DNA cross-species amplification of oriental white stork with microsatellite primers of wood stork

Eleven microsatellite loci of wood stork（Mycteria americana） were amplified in oriental white stork（Ciconia boyciana）.Six of them provided specific PCR products and other loci failed.Clone sequencing of these PCR products examine simple tandem repeat（STR） sequences in four of all six loci,while the others contained no STR.Polymorphism analysis showed that loci WSμ13 and WSμ17 were highly and moderately polymorphic loci respectively,the other two STR loci were monomorphic.In present study,the flanking sequence and the tandem repeat unit of four microsatellite loci in oriental white stork and wood stork were analyzed,the results showed the flanking sequence variations produced the significant negative effect on the cross-species amplification success rate.