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| 葡萄感霜霉病基因RAPD标记的序列分析 | |
| 引用本文： | 罗素兰,郑学勤,贺普超,王跃进.葡萄感霜霉病基因RAPD标记的序列分析[J].生物学杂志,2000,17(4):18-19. |
| 作者姓名： | 罗素兰 郑学勤 贺普超 王跃进 |
| 作者单位： | 1. 海南大学农学院 2. 热带作物生物技术国家重点实验室,海口,571101 3. 西北农业大学园艺系,杨凌,712100 |
| 基金项目： | 本研究得到热带生物技术国家重点实验室开放基金的资助 |
| 摘 要： | 利用Ｗｉｚａｒｄ ＤＮＡ ｃｌｅａｎ－ｕｐ ｓｙｓｔｅｍ纯化葡萄感霜霉病基因ＲＡＰＤ遗传标记的ＤＮＡ片段，用细菌质粒ｐＧＥＭ Ｔ－ｅａｓｙ ｖｅｃｔｏｒ克隆该片段，采用自动荧光ＤＮＡ测序仪对片段的核苷酸组成进行双向测序。来自欧洲葡萄粉红玫瑰的葡萄感霜霉病基因ＲＡＰＤ标记由８３５对核苷酸及其特定序列组成。所获的感霜霉病基因ＲＡＰＤ标记可以作为合成探针的基础，用于葡萄抗病育种过程中的早期选择及品种对霜 |
| 关 键 词： | 葡萄感霜霉病基因 RAPD标记 DNA序列 |
| 文章编号： | 1008-9632(2000)04-0018-02 |
| 修稿时间： | 2000年5月22日 |
Analysis of sequencing the RAPD marker linked to the downy mildew- susceptible genes in grapes |
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| LUO Su-lan,ZHEN Xue-qin,HE Pu-chao,WANG Yue-jin.Analysis of sequencing the RAPD marker linked to the downy mildew- susceptible genes in grapes[J].Journal of Biology,2000,17(4):18-19. | |
| Authors: | LUO Su-lan ZHEN Xue-qin HE Pu-chao WANG Yue-jin |
| Abstract: | This paper presents the sequence of RAPD marker linked to the downy mildew-susceptible genes in grapes.In grapes.The isolation DNA was purified by Wizard DNA cleanup System and inserted into plasmid pGEM T-vector easy.The sequencing was performed based upon automated fluorescent DNA sequencer(Model ABI377).The RAPD marker from the susceptible cultivar Muscat Rose (Vitis vinifera)consists of 835bp,and it's restriction-sites were analyzed using PC gene software.It is suggested that the sequence of OPO10-835 might be used as basis for synthesixing the specific PCR primers and the probe for detecting grape downy mildew-susceptibility in molecular assisted selection. |
| Keywords: | Grape downy mildew-susceptible genes RAPD marker DNA sequence| |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |