| 抗心肌肌钙蛋白T单抗杂交瘤细胞株的建立及鉴定 |
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| 引用本文： | 宋礼华,梁俊.抗心肌肌钙蛋白T单抗杂交瘤细胞株的建立及鉴定[J].生物学杂志,1999,16(6):13-14. |
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| 作者姓名： | 宋礼华 梁俊 |
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| 作者单位： | 安徽省生物研究所!合肥230031(宋礼华，杨玲，杜贤宇)，安徽省教育学院!合肥230061(梁俊，姜先荣，杨杰，傅惠英) |
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| 基金项目： | 安徽省自然科学基金!(95 —医—10) |
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| 摘 要： | 以牛心肌为原料,提取纯化得牛cTnT,并经特定处理,免疫BALB/C 小鼠,取其脾细胞与SP2/0 融合,获得两株抗cTnT 细胞3B2 和3D6 。Ig 亚类均是IgG1 。相加试验表明可识别不同表位,为建立测定cTnT方法奠定了基础。
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| 关 键 词： | 心肌肌钙蛋白T 单克隆抗体 杂交瘤 心肌梗塞 |
Establishment and analysis of monoclonal antibody against Cardiac Troponin T |
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| SONG Li-hua ,LIANG Jun ,YANG Ling ,DU Xian-yu ,JIANG Xian-rong ,YANG Jie FU Hiu-ying.Establishment and analysis of monoclonal antibody against Cardiac Troponin T[J].Journal of Biology,1999,16(6):13-14. |
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| Authors: | SONG Li-hua [] LIANG Jun [] YANG Ling [] DU Xian-yu [] JIANG Xian-rong [] YANG Jie [] FU Hiu-ying [] |
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| Institution: | SONG Li-hua [1],LIANG Jun [2],YANG Ling [1],DU Xian-yu [1],JIANG Xian-rong [2],YANG Jie [2] FU Hiu-ying [2] |
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| Abstract: | Cardiac Troponin T was purified from bovine heart,and it was specially treated before immuring Balb/c mice.Two hybtidoma cells,named 3B2 and 3D6,secreting stable mAb against cTnT were obtained with hybridoma technique.The Ig subclass of mAb was IgG1.The addition test showed that two mAb recognized different epitopes. |
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| Keywords: | Cardiac Troponin T monoclonal antibody hybridoma myocardial infarction |
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