| 从万古霉素抗性突变体中筛选碱性纤维素酶高产菌株 |
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| 引用本文： | 王丹敏,宋桂经.从万古霉素抗性突变体中筛选碱性纤维素酶高产菌株[J].生物学杂志,1999,16(4):15-16. |
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| 作者姓名： | 王丹敏 宋桂经 |
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| 作者单位： | 武警医学院微生物教研室!天津300162(王丹敏)，山东大学微生物研究所!济南250100(宋桂经) |
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| 摘 要： | 以芽胞杆菌 Ｘ—６ 为出发菌株，经甲基磺酸乙酯（ Ｅ Ｍ Ｓ） 和紫外线（ Ｕ Ｖ） 复合诱变，选育万古霉素抗性突变体。研究结果表明，抗药性突变株碱性羧甲基纤维素酶（ Ｃ Ｍ Ｃａｓｅ） 产量提高的正变率和正变幅度明显高于非抗药性菌株。从抗性突变株中获得 Ｅ Ｖ２３ 菌株，其产酶活力比出发株 Ｘ—６ 提高３２０ ％ ，酶活力达３５３ｕ／ ｍｌ。
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| 关 键 词： | EMS—UV诱变 万古霉素抗性突变体 碱性纤维素酶 |
High production strain of alkaline cellulase from vancomycine-resistant mutants of Bacillus sp.X-6 |
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| WANG Dan-min,SONG Gui-jing.High production strain of alkaline cellulase from vancomycine-resistant mutants of Bacillus sp.X-6[J].Journal of Biology,1999,16(4):15-16. |
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| Authors: | WANG Dan-min SONG Gui-jing |
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| Abstract: | Vancomycine-resistant mutants(V r m) were isolated after treatment with EMS-UV mutagensis to Bacillus sp.X-6.The production of alkaline carboxymethyl cellulae(CMCase) from the resistant mutants were obviously higher than that of no-resistant strains.A high-yield mutant(EV23) which produced 3.2 times more cellulase than the parental strain was screened from the V r m mutants.The CMCase activity redched about 3.53u/ml. |
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| Keywords: | EMS-UV mutation vancomycine-resistant mutants alkaline cellulase |
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