| 漆酶／介体体系降解黄麻纤维木质素的研究 |
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| 引用本文： | 张勇兵,董爱学,周春晓,等.漆酶／介体体系降解黄麻纤维木质素的研究[J].生物学杂志,2014(1):20-23. |
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| 作者姓名： | 张勇兵 董爱学 周春晓 等 |
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| 作者单位： | 江南大学生态纺织教育部重点实验室,江苏无锡214122 |
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| 基金项目： | 国家自然科学基金（51173071,21274055）;教育部新世纪优秀人才资助项目（NCET-12-0883）;教育部高等学校博士学科点专项科研基金资助项目（20110093110003）;教育部长江学者和创新团队发展计划（IRT1135）;江苏省自然科学基金项目（BK2011157）;江苏省“六大人才高峰”资助项目（2011-XCL-002）;江苏高校优势学科建设工程资助项目（苏政办发2011-137号） |
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| 摘 要： | 为了研究漆酶／介体处理过程中，黄麻纤维木质素结构的变化，采用二氧六环水溶液抽提制取了黄麻纤维木质素，再用漆酶／介体对其处理，通过GPC、元素分析、酚羟基含量测定、红外光谱以及核磁共振氢谱分析了漆酶／介体处理后，黄麻纤维木质素结构的变化。结果表明：经漆酶／介体处理后，黄麻纤维木质素重均分子量和数均分子量减小，酚羟基、醇羟基以及甲氧基含量降低，羰基含量增加。
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| 关 键 词： | 漆酶 黄麻纤维 木质素 降解 |
Study on degradation of jute fiber lignin by laccase and mediator system |
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| ZHANG Yong-bing,DONG Ai-xue,ZHOU Chun-xiao,YUAN Jiu-gang,WANG Qiang,FAN Xue-rong,WANG Ping,CUI Li.Study on degradation of jute fiber lignin by laccase and mediator system[J].Journal of Biology,2014(1):20-23. |
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| Authors: | ZHANG Yong-bing DONG Ai-xue ZHOU Chun-xiao YUAN Jiu-gang WANG Qiang FAN Xue-rong WANG Ping CUI Li |
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| Institution: | (Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China) |
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| Abstract: | To investigate the structural changes of jute fiber lignin during laccase and mediator system （LMS） treatment, lignins from jute fiber were extracted with dioxane/water solution, followed by LMS treatment. The structural changes of the extracted lignins from the jute fiber treated by LMS were characterized by GPC, elemental analysis, measurement of phenolic hydroxyl group content, FTIR and 1H-NMR. The resuhs suggested that the number average molecular weight and weight average molecular weight decreased after LMS treatment, while the contents of phenolic hydroxyl group, aliphatic hydroxyl group and methoxy group decreased, and the content of carbonyl group increased. |
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| Keywords: | laccase jute fiber lignin degradation |
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