| 基因工程菌发酵研究进展 |
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| 引用本文： | 张金红,陈华友,李萍萍.基因工程菌发酵研究进展[J].生物学杂志,2012(5):72-75. |
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| 作者姓名： | 张金红 陈华友 李萍萍 |
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| 作者单位： | 江苏大学现代农业装备与技术教育部重点实验室;江苏大学生命科学研究院;南京林业大学森林资源与环境学院 |
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| 基金项目： | 国家“973”资助项目(2009CB724700);国家星火计划项目(2010GA690001) |
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| 摘 要： | 基因工程菌发酵主要目标是获取高产外源基因表达蛋白。介绍并分析了基因工程菌发酵过程中表达系统、培养基、温度、pH值、溶解氧和诱导条件等因素对发酵的影响;论述了工程菌高密度培养所需的培养方式,并总结了基因工程菌发酵领域近年来的一些进展。
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| 关 键 词： | 基因工程菌 表达系统 培养条件 培养方式 |
Research progress on genetic engineering bacteria fermentation |
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| ZHANG Jin-hong,CHEN Hua-you,LI Ping-ping.Research progress on genetic engineering bacteria fermentation[J].Journal of Biology,2012(5):72-75. |
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| Authors: | ZHANG Jin-hong CHEN Hua-you LI Ping-ping |
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| Institution: | 1,3(1.Key Laboratory of Modern Agricultural Equipment and Technology,Jiangsu University,Zhenjiang 212013; 2.Institute of Life Sciences,Jiangsu University,Zhenjiang 212013; 3.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | To obtain high yield proteins encoded by the heterologous genes is the central goal for the fermentation of genetic engineering bacteria.The influences of the gene expression system,medium,temperature,pH and dissolved oxygen during the fermentation process of the engineering bacteria were described and analyzed in this paper.Subsequently,the cultivation of the high cell density culture required was discussed.Finally,the recent developments in the field of engineering bacteria fermentation were discussed and summarized. |
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| Keywords: | genetic engineering bacteria expression system culture conditions culture methods |
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